In Vivo Antioxidant and Antiulcer Activity of Parkia speciosa Ethanolic Leaf Extract against Ethanol-Induced Gastric Ulcer in RatsReport as inadecuate




In Vivo Antioxidant and Antiulcer Activity of Parkia speciosa Ethanolic Leaf Extract against Ethanol-Induced Gastric Ulcer in Rats - Download this document for free, or read online. Document in PDF available to download.

Background

The current study was carried out to examine the gastroprotective effects of Parkia speciosa against ethanol-induced gastric mucosa injury in rats.

Methodology-Principal Findings

Sprague Dawley rats were separated into 7 groups. Groups 1–2 were orally challenged with carboxymethylcellulose CMC; group 3 received 20 mg-kg omeprazole and groups 4–7 received 50, 100, 200 and 400 mg-kg of ethanolic leaf extract, respectively. After 1 h, CMC or absolute ethanol was given orally to groups 2–7. The rats were sacrificed after 1 h. Then, the injuries to the gastric mucosa were estimated through assessment of the gastric wall mucus, the gross appearance of ulcer areas, histology, immunohistochemistry and enzymatic assays. Group 2 exhibited significant mucosal injuries, with reduced gastric wall mucus and severe damage to the gastric mucosa, whereas reductions in mucosal injury were observed for groups 4–7. Groups 3–7 demonstrated a reversal in the decrease in Periodic acid-Schiff PAS staining induced by ethanol. No symptoms of toxicity or death were observed during the acute toxicity tests.

Conclusion

Treatment with the extract led to the upregulation of heat-shock protein 70 HSP70 and the downregulation of the pro-apoptotic protein BAX. Significant increases in the levels of the antioxidant defense enzymes glutathione GSH and superoxide dismutase SOD in the gastric mucosal homogenate were observed, whereas that of a lipid peroxidation marker MDA was significantly decreased. Significance was defined as p<0.05 compared to the ulcer control group Group 2.



Author: Rami Al Batran, Fouad Al-Bayaty, Mazen M. Jamil Al-Obaidi, Abdualrahman Mohammed Abdualkader, Hamid A. Hadi, Hapipah Mohd Ali, Ma

Source: http://plos.srce.hr/



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