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Flow cytometry is a versatile technique to study different aspects of the cell cycle from subpopulations of cells to detailed cell kinetic information. In this paper a basic review of cell kinetic parameters is presented followed by detailed descriptions of the different flow cytometric methodologies that can be used to extract pertinent information for a particular study. The methodologies range from simple DNA profile analysis, the use of bromodeoxyuridine to cell cycle-associated proteins such as the cyclins.

KEYWORDS

Flow Cytometry, Cell Cycle, DNA Profile, Bromodeoxyuridine, Cyclins

Cite this paper

Wilson, G. 2014 Probing the Cell Cycle with Flow Cytometry. Journal of Biomedical Science and Engineering, 7, 698-711. doi: 10.4236-jbise.2014.79069.





Author: George D. Wilson

Source: http://www.scirp.org/



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