Non-Invasive Second Harmonic Generation SHG in Macroscopy MacroSHG as Bio-Diagnosis to Image Collagen Network Organization in Extracellular MatrixReport as inadecuate




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In this work, we have considered a new multimodality imaging formacroscopy based on Second Harmonic Generation SHG method to monitorinvasivelessly the matrix collagen. As the triple helicoidally structure ofcollagen molecules appearing as not centrosymetric, very organized andspatially oriented, collagen fibrils give rise to a very strong SHG signal andcan be imaged without any exogenous dye. To integrate a multidimensional scalewith a large field of view non-sliced samples, we have adapted and validatedan instrumental coupling between a two photon excitation laser and a macroscopeto collect cartography of SHG signal. We introduced an index F-SHG based ondecay time response measured by TCSPC for respectively Fluorescence F andSecond Harmonic Generation SHG values. For various sample where proteincollagen is the major component of extracellular matrix vessel, skin, carotidevessel, rat femoral head cartilage, mouse tumor, human wharton’s jelly and rattendon or not nacre, we compared the index distribution obtained withMacroSHG. In this work, we showed for the first time that multiscale largefield imaging Macroscopy combined to Multimodality approaches SHG-TCSPCcould be an innovative and non-invasive technique to detect and identify somebiological interest molecules collagen in biomedical topics.

KEYWORDS

Multiphoton Macroscopy, Second Harmonic Generation, Collagen, Extracellular Matrix

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Dumas, D. , Werkmeister, E. , Hupont, S. , Huselstein, C. , Isla, N. , Rousseau, M. , Menu, P. and Mainard, D. 2014 Non-Invasive Second Harmonic Generation SHG in Macroscopy MacroSHG as Bio-Diagnosis to Image Collagen Network Organization in Extracellular Matrix. Engineering, 6, 485-490. doi: 10.4236-eng.2014.68050.





Author: Dominique Dumas, Elisabeth Werkmeister, Sebastien Hupont, Céline Huselstein, Natalia De Isla, Marthe Rousseau, Patrick Menu, Did

Source: http://www.scirp.org/



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