Cobalamin Analogues in Humans: A Study on Maternal and Cord BloodReport as inadecuate




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Background

Haptocorrin HC carries cobalamin analogues CorA, but whether CorA are produced in the body is unknown. All cobalamins Cbl to the foetus are delivered by the Cbl-specific protein transcobalamin TC, and therefore analysis of cord serum for CorA may help to clarify the origin of CorA.

Methods

HC-CorA were quantified in paired samples of cord serum from newborns and serum from mothers n = 69.

Results

The CorA-concentration was higher in cord serum median = 380, range: 41–780 pmol-L than in serum from the mothers median = 160, range: 64–330 pmol-L, p<0.0001. HPLC-analysis showed CorA-peaks with retention times of 13.5, 14,5 and 16.5 min in samples from both the mother and cord serum. The peak with retention time 16.5 min constituted 24% mother and 45% cord serum of the total amount CorA, and eluted as does dicyanocobinamide.

Conclusion

Our results support that CorA in the human body are derived from Cbl.



Author: Tore Forsingdal Hardlei , Rima Obeid, Wolfgang Herrmann, Ebba Nexo

Source: http://plos.srce.hr/



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