Transcriptome of the Lymantria dispar Gypsy Moth Larval Midgut in Response to Infection by Bacillus thuringiensisReport as inadecuate




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Transcriptomic profiles of the serious lepidopteran insect pest Lymantria dispar gypsy moth were characterized in the larval midgut in response to infection by Bacillus thuringiensis kurstaki, a biopesticide commonly used for its control. RNA-Seq approaches were used to define a set of 49,613 assembled transcript sequences, of which 838, 1,248 and 3,305 were respectively partitioned into high-, mid- and low-quality tiers on the basis of homology information. Digital gene expression profiles suggested genes differentially expressed at 24 hours post infection, and qRT-PCR analyses were performed for verification. The differentially expressed genes primarily associated with digestive function, including α-amylase, lipase and carboxypeptidase; immune response, including C-type lectin 4; developmental genes such as arylphorin; as well as a variety of binding proteins: cellular retinoic acid binding protein lipid-binding, insulin-related peptide binding protein protein-binding and ovary C-EBPg transcription factor nucleic acid-binding. This is the first study conducted to specifically investigate gypsy moth response to a bacterial infection challenge using large-scale sequencing technologies, and the results highlight important genes that could be involved in biopesticide resistance development or could serve as targets for biologically-based control mechanisms of this insect pest.



Author: Michael E. Sparks, Michael B. Blackburn, Daniel Kuhar, Dawn E. Gundersen-Rindal

Source: http://plos.srce.hr/



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