Protection of IFNAR −-− Mice against Bluetongue Virus Serotype 8, by Heterologous DNA-rMVA and Homologous rMVA-rMVA Vaccination, Expressing Outer-Capsid Protein VP2Report as inadecuate




Protection of IFNAR −-− Mice against Bluetongue Virus Serotype 8, by Heterologous DNA-rMVA and Homologous rMVA-rMVA Vaccination, Expressing Outer-Capsid Protein VP2 - Download this document for free, or read online. Document in PDF available to download.

The protective efficacy of recombinant vaccines expressing serotype 8 bluetongue virus BTV-8 capsid proteins was tested in a mouse model. The recombinant vaccines comprised plasmid DNA or Modified Vaccinia Ankara viruses encoding BTV VP2, VP5 or VP7 proteins. These constructs were administered alone or in combination using either a homologous prime boost vaccination regime rMVA-rMVA or a heterologous vaccination regime DNA-rMVA. The DNA-rMVA or rMVA-rMVA prime-boost were administered at a three week interval and all of the animals that received VP2 generated neutralising antibodies. The vaccinated and non-vaccinated-control mice were subsequently challenged with a lethal dose of BTV-8. Mice vaccinated with VP7 alone were not protected. However, mice vaccinated with DNA-rMVA or rMVA-rMVA expressing VP2, VP5 and VP7 or VP2 alone were all protected.



Author: Tamara Kusay Jabbar, Eva Calvo-Pinilla , Francisco Mateos, Simon Gubbins, Abdelghani Bin-Tarif, Katarzyna Bachanek-Bankowska, Oya

Source: http://plos.srce.hr/



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