The Influence of Culture Medium Type on Cellular Phenotype of Canine Adipose Derived Stem CellsReport as inadecuate




The Influence of Culture Medium Type on Cellular Phenotype of Canine Adipose Derived Stem Cells - Download this document for free, or read online. Document in PDF available to download.

Canine adipose derived stem cells ASCs hold a great promise for thetherapy of osteoarthritis in veterinary medicine. Current therapy is anautologous, stromal vascular fraction. Allogeneic ASCs provide many advantages,including efficient, cost-effective treatments while eliminating a surgicalprocedure in a diseased animal. Cultured ASCs can be expanded andcharacterized, allowing selection of desirable qualities. Use of allogeneicASCs requires selection of a culture medium that provides consistent, desirablecellular products. The supplements within a medium can greatly influencecellular phenotypes. We hypothesized that medium type influenced cellularphenotype, allowing selection of a specified cellular product for clinical applications.We evaluated ASCs derived from adipose tissue of six dogs, assessing



KEYWORDS

Canine; Mesenchymal Stem Cells; Adipose-Derived Stromal Cells; Culture Conditions

Cite this paper

Kiefer, K. , Pluhar, G. , Conzemius, M. and O’Brien, T. 2014 The Influence of Culture Medium Type on Cellular Phenotype of Canine Adipose Derived Stem Cells. Open Journal of Regenerative Medicine, 3, 28-37. doi: 10.4236-ojrm.2014.31004.





Author: Kristina M. Kiefer, G. Elizabeth Pluhar, Michael G. Conzemius, Timothy D. O’Brien

Source: http://www.scirp.org/



DOWNLOAD PDF




Related documents