Molecular and Pathogenic Study of Guignardia spp. Isolates Associated to Different HostsReport as inadecuate




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Fungi of Guignardia genusare commonly isolated from different plant species and most of the time, theyare characterized as endophytes. However, some species of this genus, like G.citricarpa and G. psidii are known as causal agents of seriousdiseases that affect important crops such as Citrus Black Spot and guava fruitrot, respectively. They are also responsible for diseases that cause foliarspots in different fruit species and also in other crops, but cause minordamages. Despite evidences that G.mangiferae colonizes different plant species, there are few studies aboutits genetic diversity associated with different hosts. This work has theobjective to characterize Guignardia isolates obtained from different hosts and tissues by RAPD, fAFLP and DNAsequence of ITS1-5.8S-ITS2 region, as well as to develop pathogenicity teststhrough cross inoculation in citrus and guava fruits. It was observed thatmolecular markers were able to discriminate isolates of different Guignardia species. Pathogenicity testsshowed that G. citricarpa caused CBSsymptoms on citrus fruits, but it did not produce any symptoms in guava fruits. G. mangiferae isolates were able tocause rot symptoms on guava fruits, but they have notproduced any symptoms on citrus fruits. Guignardia isolates obtained from mango leaves that have not been classified in species have notpresented any symptoms in citrus and guava fruits. Although G. mangiferae is commonly isolatedasymptomatically in different plants, this work supports the evidence that thisspecies has a latent pathogen behavior, at least for guava plants.



KEYWORDS

Fungi; Endophytes; Molecular Markers; Plant Disease

Cite this paper

E. Wickert, A. Souza, R. Pereira, L. Kishi, E. Lemos and A. Goes -Molecular and Pathogenic Study of Guignardia spp. Isolates Associated to Different Hosts,- Advances in Microbiology, Vol. 4 No. 2, 2014, pp. 116-125. doi: 10.4236-aim.2014.42016.





Author: Ester Wickert1*, Andressa de Souza2, Rodrigo Matheus Pereira3, Luciano Takeshi Kishi4, Eliana Gertrudes de Macedo Lemos2, Antonio

Source: http://www.scirp.org/



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