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Vitamin K2 VK2,menaquinone is a drug for osteoporosis.VK2 acts as a cofactor for γ-glutamyl carboxylase, which catalyzes thecarboxylation of specific glutamic acid residues γ-carboxylationof substrate proteins. Here we demonstrate that VK2 also regulateosteoblastgenic marker gene expression. Using VK2-immobilzed nanobeads new target proteinswere purified andidentified from osteoblastic cell line. They are prohibitin 1 and 2 PHB1 &2, respectively. To confirm the PHBs function on VK2-dependent transcription, PHB1 & 2 were knock-downand osteocalcin gene 2 transcriptions were analyzed, indicating that PHBsregulate VK2-dependent transcription. Taken together PHBs are VK2 targetproteins for osteoblastgenic transcription.

KEYWORDS

Vitamin K2; Prohibitin; Osteoblast; Runt-Related Transcription Factor 2 Runx2

Cite this paper

Uebi, T. , Umeda, M. , Maekawa, N. , Karasawa, S. , Handa, H. and Imai, T. 2013 Prohibitins, novel vitamin K2 target factors in osteoblast. Journal of Biosciences and Medicines, 1, 1-4. doi: 10.4236-jbm.2013.13001.





Author: Tatsuya Uebi, Makoto Umeda, Naoya Maekawa, Satoshi Karasawa, Hiroshi Handa, Takeshi Imai

Source: http://www.scirp.org/



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