Human Chorionic Gonadotropin β Induces Migration and Invasion via Activating ERK1-2 and MMP-2 in Human Prostate Cancer DU145 CellsReport as inadecuate




Human Chorionic Gonadotropin β Induces Migration and Invasion via Activating ERK1-2 and MMP-2 in Human Prostate Cancer DU145 Cells - Download this document for free, or read online. Document in PDF available to download.

We previously demonstrated that human chorionic gonadotropin β hCGβ induced migration and invasion in human prostate cancer cells. However, the involved molecular mechanisms are unclear. Here, we established a stable prostate cancer cell line overexpressing hCGβ and tested hCGβ-triggered signaling pathways causing cell migration and invasion. ELISA showed that the hCGβ amount secreted into medium increased with culture time after the hCGβ-transfected cells were incubated for 3, 6, 9, 12 and 24 h. More, hCGβ standards promoted MAPK ERK1-2 phosphorylation and increased MMP-2 expression and activity in both dose- and time-dependent manners in hCGβ non-transfected cells. In addition, hCGβ promoted ERK1-2 phosphorylation and increased MMP-2 expression and activity significantly in hCGβ transfected DU145 cells. Whereas ERK1-2 blocker PD98059 25 µM significantly downregulated phosphorylated ERK1-2 and MMP-2. Particularly, hCGβ promoted cell migration and invasion, yet the PD98059 diminished the hCGβ-induced cell motility under those conditions. These results indicated that hCGβ induced cell motility via promoting ERK1-2 phosphorylation and MMP-2 upregulation in human prostate cancer DU145 cells.



Author: Zongwen Li , Chunliu Li , Lianlian Du, Yan Zhou, Wei Wu

Source: http://plos.srce.hr/



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