Exponential Megapriming PCR EMP Cloning—Seamless DNA Insertion into Any Target Plasmid without Sequence ConstraintsReport as inadecuate




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We present a fast, reliable and inexpensive restriction-free cloning method for seamless DNA insertion into any plasmid without sequence limitation. Exponential megapriming PCR EMP cloning requires two consecutive PCR steps and can be carried out in one day. We show that EMP cloning has a higher efficiency than restriction-free RF cloning, especially for long inserts above 2.5 kb. EMP further enables simultaneous cloning of multiple inserts.



Author: Alexander Ulrich, Kasper R. Andersen, Thomas U. Schwartz

Source: http://plos.srce.hr/



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