Yeast Gis2 and Its Human Ortholog CNBP Are Novel Components of Stress-Induced RNP GranulesReport as inadecuate




Yeast Gis2 and Its Human Ortholog CNBP Are Novel Components of Stress-Induced RNP Granules - Download this document for free, or read online. Document in PDF available to download.

Although a CCTG expansion in the gene encoding the zinc knuckle protein CNBP causes a common form of muscular dystrophy, the function of both human CNBP and its putative budding yeast ortholog Gis2 remain poorly understood. Here we report the protein interactions of Gis2 and the subcellular locations of both Gis2 and CNBP. We found that Gis2 exhibits RNA-dependent interactions with two proteins involved in mRNA recognition, the polyA binding protein and the translation initiation factor eIF4G. We show that Gis2 is a component of two large RNA-protein granules, processing bodies and stress granules, which contain translationally repressed mRNAs. Consistent with a functional ortholog, CNBP also associates with the polyA binding protein and accumulates in stress granules during arsenite treatment of human cells. These results implicate both Gis2 and CNBP in mRNA handling during stress.



Author: Marta Rojas, George W. Farr, Cesar F. Fernandez, Laura Lauden, John C. McCormack, Sandra L. Wolin

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents