Conditional Replication Adenovirus Sensitizes A549 Cancer Cell to CisplatinReport as inadecuate




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Objective: The aim of the study was toimprove the therapeutic effect for lung cancer using a synergetic strategy ofadenovirus-based gene therapy combined with chemotherapy. Methods: A conditional replication adenovirusCRAd was employedto treat the A549 lung cancer cells and cisplatin-resisted A549A549-DDP cells.In vitro MTS - PMS assay were used to evaluatethe cell viability. PCR were used to detect expression of Coxsackie receptor CARand Multidrug resistanceMDR gene. The invivo anti-tumor effect of CRAd and cisplatin was evaluated using asubcutaneous mouse model. Results: The CRAd sensitizes A549 cancer cells tocisplatin. The mechanism of enhanced cell growth inhibition is associated with theincreased CAR and MDR expression. Conclusion: Our approach is better than theconventional gene therapy and chemotherapy strategy.

KEYWORDS

Cisplatin; Adenovirus; Lung Cancer

Cite this paper

Y. Liu and Y. Huang -Conditional Replication Adenovirus Sensitizes A549 Cancer Cell to Cisplatin,- Engineering, Vol. 4 No. 10B, 2012, pp. 165-166. doi: 10.4236-eng.2012.410B043.





Author: Yanan Liu, Yinghui Huang

Source: http://www.scirp.org/



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