The omp2 Gene of HPS-type Bacteria Cloning and Sequence Analysis Isolates from Sichuan ProvinceReport as inadecuate




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In order to compare thehomology and antigen of Haemophilus parasuis HPS4 outer membrane protein P2omp2, we design a test with specific primers, using PCR amplification of isolates of Haemophilus parasuis from Sichuan ProvinceHPSch2010, ompP2 gene will be cloned into the pGM-T vector, and transformed intoE. coli DH5α. Identified by PCR and sequencing and analysis, the sequencingresults showed that the published 4 HPS SW124 strains omp2 gene 1077bp, compared with the amplified 1086bp purposefragmentcontaining omp2 genes, is relatively stable, with the nucleotidehomology level 97% and amino acid homology level of 92.5%. Thevariable regions are mainly concentrated in the three base sequences:40-65,110-156,180-202.

KEYWORDS

Haemophilus parasuis, virulence genes, omp2

Cite this paper

L. Li, Z. Yang, Y. Wang, Q. Jin, X. Wu and R. Zhang -The omp2 Gene of HPS-type Bacteria Cloning and Sequence Analysis Isolates from Sichuan Province,- Engineering, Vol. 4 No. 10B, 2012, pp. 153-158. doi: 10.4236-eng.2012.410B040.





Author: Lirui Li, Zexiao Yang, Yin Wang, Qiu Jin, Xulong Wu, Rongchang Zhang

Source: http://www.scirp.org/



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