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Inducing Mesenchymalstem cells to differentiate into cardiomyocycte-like cells and endothelialprogenitor cells orientedly and evaluating the curative effect of theassociated cell transplantation on the rabbit myocardial infarction MI. Methods: Mesenchymal stem cells MSCswere isolated from the bone marrow of 24 rabbits and cultured in special cellculture medium containing 5-azacytidine5-AZA, endothelial cell growth supplements ECGS, vascular endothelialgrowth factor VEGF and basic fibroblast growthfactor BFGF respectively. The cell transplantation was performed 2weeks after MI. Rabbits were divided into control group, cardiomyocytes-likecell group, endothelial progenitor cell groupand combination group. We used the echocardiography to measure the heartfunction 2 to 4 weeks after MI, TTC to measure the area of the infarction, flowcytometry to estimate the cell apoptosis. Results: After induced, MSCs were differentiated orientedly into cardiomyocycte-like cells CLCsand endothelial progenitor cells EPCs. CLCs became greater and had a -stick-or -ball- shape. Transmisson electron microscopy showed that the cells had ovalnuclei positioned in the central part andwell organized myofilaments, atrial granules and mitochomdrion. RT-PCRshowed the expression of the atrial natriuretic polypeptide, phospholamban andmyosin heavy chain in CLCs. EPCs formed confluent one-celled layer which showeda cobblestone shape by phase-contrast microscope. The expression of CD133 inEPCs was much at first and then descended gradually. Compared with the controlgroup, cell transplantation could improve the heart function, reduce the sizeof MI, decrease the left ventricular end systole diameter and end diastolicdiameter, suppressed cell apoptosis. The curative effect of cell transplantationwas better in the associated-cell group thanin the single-cell transplantation group LVEF: 32.49% ± 1.29% vs 53.22% ± 2.13% vs 56.91% ± 2.04% vs62.61% ± 2.37%, P < 0.05; LVESD: 1.23 ± 0.02 vs0.98 ± 0.04 vs 0.98 ± 0.12 vs 1.11 ± 0.03, P < 0.05;LVEDD: 1.53 ± 0.13 vs 1.24 ± 0.02 vs 1.21 ± 0.09vs 1.01 ± 0.01, P < 0.05; the area of infarction: 35.17% ± 0.98% vs 28.61% ± 1.24% vs 29.73% ± 2.11% vs 22.82%± 3.12%, P <0.05; apotosis: 8.6% ± 0.94% vs 6.94% ± 0.59% vs 6.4% ± 0.27% vs 4.63% ±0.74%, P < 0.05. Conclusions: This study showed that MSCs can differentiate into CLCs and EPCs in the given conditions and the associated cell transplantationis better than the single cell transplantationto treat MI.

KEYWORDS

Mesenchymal Stem Cell; Cardiomyocycte-Like Cell; Endothelial Progenitor Cell; Cell Transplantation

Cite this paper

Fang, Z. , Zhou, C. , Zheng, X. , Liu, B. , Chen, L. , Shen, C. , Liu, P. and Huang, Y. 2013 The curative effect of the associated cell transplantation on the rabbit myocardial infarction. Stem Cell Discovery, 3, 203-210. doi: 10.4236-scd.2013.34025.





Author: Zhicheng Fang, Chang’e Zhou, Xiang Zheng, Boyi Liu, Li Chen, Chunfeng Shen, Pei Liu, Yunfei Huang

Source: http://www.scirp.org/



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