Carbon utilization profile of a thermophilic fungus, Thermomyces lanuginosus using phenotypic microarrayReport as inadecuate




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The thermophilic filamentousfungus, Thermomyces lanuginosus produces the largest amount of xylanase reported. In addition to this, itexpresses large amount of other enzymes that have been used industrially orhave academic interest. Thus, this fungus has a potential to be applied forbiomass conversion to produce biofuel or other applications. In this study, theBiolog system was used to characterize the utilisation and growth of T. lanuginosus on 95 carbon sources. Thecarbohydrates based compounds, both single sugars and oligosaccharide, showedthe best utilisation profile, with the pentose sugar xylose inducing thehighest growth, followed by trehelose, raffinose, D-mannose turanose fructoseand glucose. Among oligosaccharides, sucrose had the highest myceliumformation followed by stachyose, maltose, maltotriose, glycogen and dextrin.Interestingly the fungus also grew well on cellobiose suggesting that thisfungus can produce cellulose hydrolysing proteins. D-alanine was the bestamino acid to promote fungal growth while the effect of other amino acidstested was similar to the control. These results demonstrate the ability ofthis fungus to grow relatively well on most plant based compounds thus makingthis fungus a possible candidate for plant biomass conversion which can beapplied to a number of biotechnological applications including biofuel production.

KEYWORDS

Filamentous Fungi; Thermophilic; Carbon Source; Hexose; Pentose

Cite this paper

Mchunu, N. , Permaul, K. , Alam, M. and Singh, S. 2013 Carbon utilization profile of a thermophilic fungus, Thermomyces lanuginosus using phenotypic microarray. Advances in Bioscience and Biotechnology, 4, 24-32. doi: 10.4236-abb.2013.49A004.





Author: Nokuthula Peace Mchunu, Kugen Permaul, Maqsudul Alam, Suren Singh

Source: http://www.scirp.org/



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