Cell Origami: Self-Folding of Three-Dimensional Cell-Laden Microstructures Driven by Cell Traction ForceReport as inadecuate




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This paper describes a method of generating three-dimensional 3D cell-laden microstructures by applying the principle of origami folding technique and cell traction force CTF. We harness the CTF as a biological driving force to fold the microstructures. Cells stretch and adhere across multiple microplates. Upon detaching the microplates from a substrate, CTF causes the plates to lift and fold according to a prescribed pattern. This self-folding technique using cells is highly biocompatible and does not involve special material requirements for the microplates and hinges to induce folding. We successfully produced various 3D cell-laden microstructures by just changing the geometry of the patterned 2D plates. We also achieved mass-production of the 3D cell-laden microstructures without causing damage to the cells. We believe that our methods will be useful for biotechnology applications that require analysis of cells in 3D configurations and for self-assembly of cell-based micro-medical devices.



Author: Kaori Kuribayashi-Shigetomi, Hiroaki Onoe, Shoji Takeuchi

Source: http://plos.srce.hr/



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