Effects of 5-aza-2’-deoxyctidine on the development of porcine parthenogenetic and nuclear transfer embryosReport as inadecuate




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The current study was to investigate whether embryo or fetal fibroblast cells treated with 5-aza-2’-deoxyctidine 5-aza-dC have a positive effect on the in vitro development of porcine parthenogenetic and cloned embryos. To this end, porcine fetal fibroblast cells were treated with different concentrations 5 nM, 50 nM and 500 nM of 5-aza-dC for different exposure times 1, 6 and 20 hours, the results showed that DNA methylation in PRE-1 SINE region was gradually reduced over time in cells treated with 5-aza-dC. To determine the effect of 5-aza-dC on in vitro development of porcine activated oocytes, the parthenogenetic embryo was treated with 5-aza- dC. Notably, treatment with 5 nM 5-aza-dC for 1 hour led to a significant improvement in blastocyst development, compared with the control group. The effects of donor cell treatment with 5-aza-dC on porcine cloned embryos development were further examined by treating fetal fibroblast cells with various concentrations 5 nM, 50 nM and 500 nM of 5-aza-dC for different exposure times 1, 6 and 20 hours. Exposure of cells in 5 nM 5-aza-dC for 1 - 20 hours led to a significant improvement in the percentage of developed blastocysts, while treatment with 500 nM 5-aza-dC did not affect blastocyst development, compared to untreated controls. These findings indicate that treatment of fetal fibro-blast cells with relatively low concentrations of 5-aza-dC for short exposure times improves subsequent blastocyst development of porcine cloned embryos.

KEYWORDS

5-aza-2’-dC; DNA Methylation; Parthenogenetic Embryo; Clone Embryo; In Vitro Development

Cite this paper

Diao, Y. , Naruse, K. , Li, X. , Han, R. , Kim, D. , Lin, T. and Jin, D. 2013 Effects of 5-aza-2’-deoxyctidine on the development of porcine parthenogenetic and nuclear transfer embryos. Natural Science, 5, 31-37. doi: 10.4236-ns.2013.57A005.





Author: Yun Fei Diao, Kenji Naruse, Xiao Xia Li, Rong Xun Han, Dong Kyo Kim, Tao Lin, Dong II Jin

Source: http://www.scirp.org/



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