Single sperm selection and DNA fragmentation analysis: The case of MSOME-IMSIReport as inadecuate




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Sperm of poor quality may affect syngamy after fertilization, embryo development up to the blastocyst stage and reproductive outcome. Subsequently, sperm selection based on morphological characteristics and sperm DNA quality may help to partially avoid these problems. Today, highly efficient sperm selection based on morphological characteristics can be attained using the motile sperm organelle morphology MSOME examination, and the spermatozoa selected can be used for ICSI through a fertilization strategy known as intra-cytoplasmic morphologically selected sperm injection IMSI. The aim of this investigation was to develop a simple methodology to assess sperm DNA fragmentation in single spermatozoa following MSOME- IMSI, to test the hypothesis that morphologically normal spermatozoa, with an absence of vacuolization, is free of DNA damage. The results indicated that MSOME-IMSI-selected sperm, combined with the Sperm Chromatin Dispersion test SCD; Oligo-Halosperm, can be reliably used to assess sperm DNA damage in selected single spermatozoa 75% average efficiency, thereby establishing a direct relationship between a good morphological pattern on the sperm and a good DNA quality. Furthermore, results showed spermatozoa presenting a normal morphology and no traces of vacuolization to be fully free of DNA damage. However, traces of vacuolization and more severe morphological alterations were accompanied by significant increases in the proportion of sperm containing a damaged DNA molecule. Interestingly, subtle morphological differences observed between normal and non-vacuolated and normal but vacuolated sperm exhibited significant differrences in the ability of the SCD-Oligo-Halosperm treated sperm to expand DNA fibers following protein depletion.

KEYWORDS

MSOME-IMSI; Assisted Reproductive Technology; Fertility; Sperm DNA Fragmentation; Male Factor; Semen Processing

Cite this paper

Gosálvez, J. , Migueles, B. , López-Fernández, C. , Sanchéz-Martín, F. and Sáchez-Martín, P. 2013 Single sperm selection and DNA fragmentation analysis: The case of MSOME-IMSI. Natural Science, 5, 7-14. doi: 10.4236-ns.2013.57A002.





Author: Jaime Gosálvez, Beatriz Migueles, Carmen López-Fernández, Fernando Sanchéz-Martín, Pascual Sáchez-Martín

Source: http://www.scirp.org/



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