Usefulness of Basic Fibroblast Growth Factor bFGF Loaded Dissolving Microneedles for Local Therapy of Skin WoundsReport as inadecuate




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Purpose: The usefulness of dissolving microneedles DMs for local skin therapy by basic fibroblast growth factor bFGF was studied in rats. Methods: We prepared four kinds of bFGF-loaded DMs, approximately 500 μm length and 300 μm diameter at the bottom. Long-term stability and dissolution studies were performed by HPLC method. Pharmacokinetic and pharmacological evaluations were performed after administration of bFGF loaded DMs to rats. Results: The bFGF contents were 2.15 ± 0.07, 1.07 ± 0.04, 0.56 ± 0.07 and 0.12 ± 0.03 μg. The 100.2 ± 3.4%, 100.2 ± 3.3%, 99.3 ± 1.4% and 100.4 ± 3.0% of bFGF were recovered after 1, 3 and 6 months and 1 year incubation at 40°C. The bFGF was released from DMs within 5 min. In a pharmacokinetic study using 2.0 and 1.0 μg bFGF-loaded DMs, no systemic exposure of bFGF was detected. The initial bFGF concentrations in the rat skin tissue after administration of bFGF-loaded DMs to the hair-removed rat abdominal skin were 510.2 ± 20.1 ng-g wet weight for 2 μg bFGF DMs and 264.2 ± 56.5 ng-g wet weight for 1 μg DMs, declining slowly thereafter to 226.3 ± 33.5 and 105.1 ± 27.4 ng-g wet weight at 6 hr after administration. Good dose-dependency was observed. Pharmacological evaluation of bFGF-loaded DMs of 2.0, 1.0, 0.5, and 0.1 μg, in the wound healing rat model, all used DMs, but 0.1 μg DMs, showed good healing effects. Considered collectively, these results suggest the usefulness of bFGF-loaded DMs for local therapy of skin wound disease.

KEYWORDS

Basic Fibroblast Growth Factor bFGF; Dissolving Microneedles DMs; Transdermal Delivery;Wound Healing; Rats

Cite this paper

K. Takada, Y. Ito, K. Matsumoto, Y. Sato, M. Nishio, Y. Tadano, Y. Kamei, Y. Takemura, N. Inoue, Y. Akasaka and I. Ono -Usefulness of Basic Fibroblast Growth Factor bFGF Loaded Dissolving Microneedles for Local Therapy of Skin Wounds,- Journal of Biomaterials and Nanobiotechnology, Vol. 4 No. 3, 2013, pp. 256-264. doi: 10.4236-jbnb.2013.43032.





Author: Kanji Takada, Yukako Ito, Kengo Matsumoto, Yuka Sato, Maimi Nishio, Yurie Tadano, Yuri Kamei, Yutaro Takemura, Nana Inoue, Yoshik

Source: http://www.scirp.org/



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