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ELL2 eleven-nineteenlysine-rich leukemia transcription elongation factor, a component of a largercomplex with pTEFb cyclin T and CDK9 and AF4, is up-regulated in plasma cellswhere it influences mRNA processing by increasing exon skipping and enhancingproximal poly A site use. ELL2 is needed to produce the secretory-specific Igheavy chain mRNA while ELL1 mRNA does not change in abundance with B cellstages. To investigate the potential interactions of other proteins with theELL1 and ELL2 proteins, we preformed yeast two-hybrid studies. HSP40 andTestin were found to bind to ELL2 in its amino-terminal half. PCNA binds toELL2 in a region encompassing amino acids 186 - 344. The potent transcriptionfactors HIF1 α and ZNF622 interact with both ELL1and 2 in the central, proline rich region. Meanwhile, BBS2 and ING3 interactwith ELL1 but not ELL2 in this central proline-rich region. Many of theELL-interacting-proteins uncovered in the two-hybrid screen are tumoursuppressors that may work through the ELL: pTEFb complex to suppressor activate sets of genes in plasma cells.

KEYWORDS

Transcription Elongation; Immunoglobulin Synthesis; Yeast Two-Hybrid System

Cite this paper

Arumemi, F. , Bayles, I. , Paul, J. and Milcarek, C. 2013 Shared and discrete interacting partners of ELL1 and ELL2 by yeast two-hybrid assay. Advances in Bioscience and Biotechnology, 4, 774-780. doi: 10.4236-abb.2013.47101.





Author: Fortuna Arumemi, Ian Bayles, Joshua Paul, Christine Milcarek

Source: http://www.scirp.org/



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