Tissue-Specific Distribution of Secondary Metabolites in Rapeseed Brassica napus L.Report as inadecuate




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Four different parts, hypocotyl and radicle HR, inner cotyledon IC, outer cotyledon OC, seed coat and endosperm SE, were sampled from mature rapeseed Brassica napus L. by laser microdissection. Subsequently, major secondary metabolites, glucosinolates and sinapine, as well as three minor ones, a cyclic spermidine conjugate and two flavonoids, representing different compound categories, were qualified and quantified in dissected samples by high-performance liquid chromatography with diode array detection and mass spectrometry. No qualitative and quantitative difference of glucosinolates and sinapine was detected in embryo tissues HR, IC and OC. On the other hand, the three minor compounds were observed to be distributed unevenly in different rapeseed tissues. The hypothetic biological functions of the distribution patterns of different secondary metabolites in rapeseed are discussed.



Author: Jingjing Fang, Michael Reichelt, William Hidalgo, Sara Agnolet, Bernd Schneider

Source: http://plos.srce.hr/



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