Distribution and Possible Function of Cannabinoid Receptor Subtype 1 in the Human ProstateReport as inadecuate




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Background: Cannabinoid receptor subtype 1CB1 has a relationship to the proliferation of various cells includingmalignant tumoral cells. We investigated and compared the expression of CB1 in benign and malignant human prostatetissues and in benign and malignant human prostate cell lines, as well as itsfunction for the proliferation of human prostate cancer cells. Methods: Real-time quantitative PCR was performed to compare its expressions in humanprostate tissues normal, benign hyperplasia, and cancer and prostate cell lines3 normal and 3 malignant. For localization of CB1, immunofluorescent staining with rabbit anti-CB1 polyclonalantibodies and tetramethyl isothiocyanate TRITC-labeled swine anti-rabbitimmunoglobulin DAKO were used under fluorescence microscope. Tofurther analyze whether cell death was induced by anandamide non-selectiveagonist for CB1-CB2 via a receptor dependent mechanism, the viability of DU145 cells,which is known as androgen-insensitive prostate cancer cell, was measured usingMTT assay. Results: CB1mRNA was found to be expressed in the all 3human prostate tissues, however, CB1 protein was expressed in BPH and low grademalignant PC tissues, but not in high grade malignant PC tissues. CB1 as forcell lines, the expression of CB1 was low in malignant cell lines except for DU145.Anandamide elicited cell death, which was significantly inhibited by AM251selective antagonist for CB1, indicating that cell death induced by anandamide in DU145 cells was mediated by CB1. Anandamide time-dependently elicits up-regulation of CB1 in DU145 cells. Conclusions: CB1may be an inhibitory regulator of androgen-insensitive human prostate cancerepithelial cell growth.

KEYWORDS

Prostate Cancer; Prostate Cell; Cannabinoid Receptor; CB1

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M. Kamiyama, M. Fukasawa, Y. Takihana, N. Sawada, H. Nakagomi, M. Yoshiyama, I. Araki and M. Takeda -Distribution and Possible Function of Cannabinoid Receptor Subtype 1 in the Human Prostate,- Open Journal of Urology, Vol. 3 No. 2, 2013, pp. 102-109. doi: 10.4236-oju.2013.32020.





Author: Manabu Kamiyama, Mizuya Fukasawa, Yoshio Takihana, Norifumi Sawada, Hiroshi Nakagomi, Mitsuharu Yoshiyama, Isao Araki, Masayuki T

Source: http://www.scirp.org/



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