Enzymatic activities and kinetic properties of β-glucosidase from selected white rot fungiReport as inadecuate




Enzymatic activities and kinetic properties of β-glucosidase from selected white rot fungi - Download this document for free, or read online. Document in PDF available to download.

Beta-glucosidase is among the suite of enzymes producedby white rot fungi WRF to biodegrade plant biomass. This study investigatedthe enzymatic activities and kinetic properties of β-glucosidase from seventeen WRF comprised of the followingspecies from various geographical locations: Pleurotus ostreatus, Auricularia auricular, Polyporussquamosus, Trametes versicolor, Lentinula edodes, and Grifola frondosa. All the WRF studiedshowed β-glucosidase activities.Significant variations in protein and carbohydrate contents were also recorded.Beta-glucosidase activities after 30 min of incubation ranged from 6.4 μg T. versicolor to 225 μg G. frondosa. The calculated kineticconstant Km ranged from0.47 μM A. auricular-1120 to 719 μM L.edodes-7. The Vmax depending on the kinetic transformation model ranged from 0.21 μg·min-1 T. versicolor to 9.70 μg·min-1 G. frondosa-28. Beta-glucosidaseactivities also exhibited pH optima between 3.5 and 5.0 while temperatureoptima were between 60°C and 70°Cwith some media exhibiting a secondary temperature peak at 90°C attributable tothe presence of thermostable isoenzyme. WRF if appropriately screened and purified can be harnessed to potentially improve the bio-conversion of celluloseto glucose and also facilitate efficient plant biomass biodegradation and production of useful plant bio-products.

KEYWORDS

White Rot Fungi; β-Glucosidase; EC 3.2.1.21; Enzymatic Activities; Kinetic Properties; Plant Biomass

Cite this paper

Mfombep, P. , Senwo, Z. and Isikhuemhen, O. 2013 Enzymatic activities and kinetic properties of β-glucosidase from selected white rot fungi. Advances in Biological Chemistry, 3, 198-207. doi: 10.4236-abc.2013.32025.





Author: Priscilla M. Mfombep, Zachary N. Senwo, Omoanghe S. Isikhuemhen

Source: http://www.scirp.org/



DOWNLOAD PDF




Related documents