Using of In-Situ Mercury Film Sensor Hyphenated with Affinity Voltammetry for High Throughput Drug-Protein Binding StudiesReport as inadecuate




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A new simple and reliable in-situ mercury film sensor coupled withaffinity differential pulse stripping voltammetry ADSPV or affinity cyclicvoltammetry ACV was investigated. The interaction of fenoprofen with bovineserum albumin BSA onto the proposed electrochemical sensor was studied.The nature of the electrochemical process of fenoprofen by cyclicvoltammetry was depicted. Reproducibility of the proposed method was checkedgiving a precision of 0.073 standard deviation. The limit of detection andlimit of quantification were 7.0 and 22.0 nmol-L, respectively. Fenoprofen wasinteracted with BSA by 1:1 stoichiometry to form electroinactive supramolecularcomplex. The binding constant was precisely estimated by non-linear regressionanalysis based on the shifting of analyte peak potentials. The proposedexperiments and data analysis could be used to investigate the drug-protein bindingconstant within a short analysis time compared to other chromatographictechniques.

KEYWORDS

Sensor; Affinity Voltammetry; Fenoprofen; Bovine Serum Albumin; Binding Constant

Cite this paper

A. Youssef and D. El-Hady -Using of In-Situ Mercury Film Sensor Hyphenated with Affinity Voltammetry for High Throughput Drug-Protein Binding Studies,- American Journal of Analytical Chemistry, Vol. 4 No. 4, 2013, pp. 159-165. doi: 10.4236-ajac.2013.44021.





Author: Ahmed K. Youssef, Deia Abd El-Hady

Source: http://www.scirp.org/



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