Green tea extract and epigallocatechin 3-gallate reduced labile iron pool and protected oxidative stress in iron-loaded cultured hepatocytesReport as inadecuate




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Cellular and mitochondrial damage can be caused by labile iron pool LIP and mediated by reactive oxygen species ROS. Livers of the thalassemias have highly increased levels of LIP and ROS. Green tea extract GTE and epigallocatechin 3-gallatte EGCG can potentially protect liver inflammation, fibrosis and cancer due to their anti-oxidative and iron-chelating activities. We studied the effects of GTE and EGCG on intracellular LIP and ROS, and mitochondrial membrane potential ΔΨm in mouse hepatocyte and HepG2 cell cultures using specific fluorescent techniques. Treatment with GTE 12.5 - 25 mg-dl and EGCG 25 - 50 μM significantly lowered levels of ΔΨm in the mouse hepatocytes; however, combined treatment of 25 μM DFP with GTE and EGCG did not enhance the decrease of hepatic ΔΨm. The results showed that GTE and EGCG effectively removed the intracellular LIP and ROS, and relieved the mitochondria membrane collapse of the liver cells, suggesting a hepatoprotective effect of green tea extract and EGCG in the hepatocytes with iron overload. Their actions might be related to iron-chelating and free radical-scavenging capacities. Whether the effects can improve iron overload and oxidative stress in thalassemia patients remains to be seen upon further examination.

KEYWORDS

Green Tea; Epigallocatechin Gallate; Hepatocytes; Labile Iron Pool; Iron; Reactive OxygenSpecies

Cite this paper

Srichairatanakool, S. , Kulprachakarn, K. , Pangjit, K. , Pattanapanyasat, K. and Fuchaeron, S. 2012 Green tea extract and epigallocatechin 3-gallate reduced labile iron pool and protected oxidative stress in iron-loaded cultured hepatocytes. Advances in Bioscience and Biotechnology, 3, 1140-1150. doi: 10.4236-abb.2012.38140.





Author: Somdet Srichairatanakool, Kanokwan Kulprachakarn, Kanjana Pangjit, Kovit Pattanapanyasat, Suthat Fuchaeron

Source: http://www.scirp.org/



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