Characterization of the Che7 System of Myxococcus xanthusReport as inadecuate

Characterization of the Che7 System of Myxococcus xanthus

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Myxococcus xanthus is a Gram negative soil bacterium that utilizes eightchemosensory systems of interacting proteins, most of which are homologous to the E.colichemotaxis proteins, in order to regulate its two types of gliding motility A and S,predation of other organisms, and multicellular fruiting body formation. The M. xanthusChe7 system, which has not been thoroughly characterized to date, is of specific interestbecause it is thought to regulate A motility through exopolysaccharide EPS production,contain a completely cytoplasmic Mcp methyl-accepting chemotaxis protein, and havetwo functioning non-chemotaxis proteins: Cpc7 and Des7. In this study, we explored theprotein-protein interactions of the Che7 system of M. xanthus through yeast two-hybridand beta-galactosidase assays. In addition to the expected interactions between the Che7chemotaxis homologs, we found that Cpc7 interacted with both Des7 and Mcp7. Thissuggests that Cpc7 is cytoplasmic and that several possibilities for the mechanism of signaltransduction in the Che7 system of M. xanthus exist. From these results, we propose a newprotein interaction model for the Che7 chemosensory two-component signal transductionsystem.

Undergraduate Research Option Theses - School of Biology Undergraduate Research Option Theses -

Author: Epperson, Erin - Kirby, John - -


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