Endothelial Cells Obtained from Patients Affected by Chronic Venous Disease Exhibit a Pro-Inflammatory PhenotypeReport as inadecuate




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Background

The inflammatory properties of vein endothelium in relation to chronic venous disease CVD have been poorly investigated. Therefore, new insights on the characteristics of large vein endothelium would increase our knowledge of large vessel physiopathology.

Methodology-Principal Findings

Surgical specimens of veins were obtained from the tertiary venous network R3 and-or saphenous vein SF of patients affected by CVD and from control individuals. Highly purified venous endothelial cell VEC cultures obtained from CVD patients were characterized for morphological, phenotypic and functional properties compared to control VEC. An increase of CD31-PECAM-1, CD146 and ICAM-1 surface levels was documented at flow cytometry in pathological VEC with respect to normal controls. Of note, the strongest expression of these pro-inflammatory markers was observed in VEC obtained from patients with more advanced disease. Similarly, spontaneous cell proliferation and resistance to starvation was higher in pathological than in normal VEC, while the migratory response of VEC showed an opposite trend, being significantly lower in VEC obtained from pathological specimens. In addition, in keeping with a higher baseline transcriptional activity of NF-kB, the release of the pro-inflammatory cytokines osteoprotegerin OPG and vascular endothelial growth factor VEGF was higher in pathological VEC cultures with respect to control VEC. Interestingly, there was a systemic correlation to these in vitro data, as demonstrated by higher serum OPG and VEGF levels in CVD patients with respect to normal healthy controls.

Conclusion-Significance

Taken together, these data indicate that large vein endothelial cells obtained from CVD patients exhibit a pro-inflammatory phenotype, which might significantly contribute to systemic inflammation in CVD patients.



Author: Veronica Tisato, Giorgio Zauli, Rebecca Voltan, Sergio Gianesini, Maria Grazia di Iasio, Ilaria Volpi, Guido Fiorentini, Paolo Za

Source: http://plos.srce.hr/



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