Increased resistance to apoptosis during differentiation and syncytialization of BeWo choriocarcinoma cellsReport as inadecuate




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Transition from mononuclear villous cytotrophoblast into multinuclear syncytiotrophoblast in the human placenta is accompanied by changes in apoptosis-related proteins and an apparent increased resistance to induced apoptosis. We investigated the specific nature and timing of changes in Bcl-2, Bax, p53, and caspases 3 and 8 in forskolin-treated BeWo choriocarcinoma cells, a model for villous cytotrophoblast differentiation. BeWo cells were treated with forskolin or vehicle alone for up to 72 h and evaluated at 24 h intervals for syncytialization and quantitative expression specific apoptosis-related proteins and mRNAs. Syncytialization was quantified using fluorescent staining of intercellular membranes and enumeration of the percentage of nuclei in multinucleate cells, and differential localization of apoptosis-related proteins to multinuclear or mononuclear cells was determined by quantitative immunofluorescence. Forskolin treatment for up to 72 h resulted in 80% syncytialization, increased expression of Bcl-2 protein P < 0.01 and mRNA P < 0.05, and significantly decreased expression of protein and mRNA for Bax, p53, and caspases 3 and 8. Syncytialized cells expressed higher levels of Bcl-2 protein concurrent with increased resistance to cisplatin-induced apoptosis. Thus, syncytialization of BeWo cells was accompanied by altered transcription of apoptotic-related proteins characteristic of increased apoptosis resistance secondary to increased expression of the anti-apoptotic protein Bcl-2 and diminish expression of pro-apoptotic proteins.

KEYWORDS

BeWo; Trophoblast; Placenta; Caspase 8; Caspase 3; Bcl-2; Intercellular Fusion

Cite this paper

Wei, B. , Xu, C. and Rote, N. 2012 Increased resistance to apoptosis during differentiation and syncytialization of BeWo choriocarcinoma cells. Advances in Bioscience and Biotechnology, 3, 805-813. doi: 10.4236-abb.2012.326100.





Author: Bih-Rong Wei, Chuan Xu, Neal S. Rote

Source: http://www.scirp.org/



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