Exposure of Phosphatidylserine on Leishmania amazonensis Isolates Is Associated with Diffuse Cutaneous Leishmaniasis and Parasite InfectivityReport as inadecuate




Exposure of Phosphatidylserine on Leishmania amazonensis Isolates Is Associated with Diffuse Cutaneous Leishmaniasis and Parasite Infectivity - Download this document for free, or read online. Document in PDF available to download.

Diffuse cutaneous leishmaniasis DCL is a rare clinical manifestation of leishmaniasis, characterized by an inefficient parasite-specific cellular response and heavily parasitized macrophages. In Brazil, Leishmania Leishmania amazonensis is the main species involved in DCL cases. In the experimental model, recognition of phosphatidylserine PS molecules exposed on the surface of amastigotes forms of L. amazonensis inhibits the inflammatory response of infected macrophages as a strategy to evade the host immune surveillance. In this study, we examined whether PS exposure on L. amazonensis isolates from DCL patients operated as a parasite pathogenic factor and as a putative suppression mechanism of immune response during the infection. Peritoneal macrophages from F1 mice BALB-c×C57BL-6 were infected with different L. amazonensis isolates from patients with localized cutaneous leishmaniasis LCL or DCL. DCL isolates showed higher PS exposure than their counterparts from LCL patients. In addition, PS exposure was positively correlated with clinical parameters of the human infection number of lesions and time of disease and with characteristics of the experimental infection macrophage infection and anti-inflammatory cytokine induction. Furthermore, parasites isolated from DCL patients displayed an increased area in parasitophorous vacuoles PV when compared to those isolated from LCL patients. Thus, this study shows for the first time that a parasite factor exposed PS might be associated with parasite survival-persistence in macrophages and lesion exacerbation during the course of DCL, providing new insights regarding pathogenic mechanism in this rare chronic disease.



Author: Jaqueline França-Costa , João Luiz Mendes Wanderley , Poliana Deolindo, Jessica B. Zarattini, Jackson Costa, Lynn Soong, Marcel

Source: http://plos.srce.hr/



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