Identification of Genes Related to White and Black Plumage Formation by RNA-Seq from White and Black Feather Bulbs in DucksReport as inadecuate




Identification of Genes Related to White and Black Plumage Formation by RNA-Seq from White and Black Feather Bulbs in Ducks - Download this document for free, or read online. Document in PDF available to download.

To elucidate the genes involved in the formation of white and black plumage in ducks, RNA from white and black feather bulbs of an F2 population were analyzed using RNA-Seq. A total of 2,642 expressed sequence tags showed significant differential expression between white and black feather bulbs. Among these tags, 186 matched 133 annotated genes that grouped into 94 pathways. A number of genes controlling melanogenesis showed differential expression between the two types of feather bulbs. This differential expression was confirmed by qPCR analysis and demonstrated that Tyr Tyrosinase and Tyrp1 Tyrosinase-related protein-1 were expressed not in W-W white feather bulb from white dorsal plumage and W-WB white feather bulb from white-black dorsal plumage but in B-B black feather bulb from black dorsal plumage and B-WB black feather bulb from white-black dorsal plumage feather bulbs. Tyrp2 Tyrosinase-related protein-2 gene did not show expression in the four types of feather bulbs but expressed in retina. C-kit The tyrosine kinase receptor expressed in all of the samples but the relative mRNA expression in B-B or B-WB was approximately 10 fold higher than that in W-W or W-WB. Additionally, only one of the two Mitf isoforms was associated with plumage color determination. Downregulation of c-Kit and Mitf in feather bulbs may be the cause of white plumage in the duck.



Author: Shijun Li, Cui Wang, Wenhua Yu, Shuhong Zhao, Yanzhang Gong

Source: http://plos.srce.hr/



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