Selection of Reliable Reference Genes in Caenorhabditis elegans for Analysis of NanotoxicityReport as inadecuate




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Despite rapid development and application of a wide range of manufactured metal oxide nanoparticles NPs, the understanding of potential risks of using NPs is less completed, especially at the molecular level. The nematode Caenorhabditis elegans C.elegans has been emerging as an environmental model to study the molecular mechanism of environmental contaminations, using standard genetic tools such as the real-time quantitative PCR RT-qPCR. The most important factor that may affect the accuracy of RT-qPCR is to choose appropriate genes for normalization. In this study, we selected 13 reference gene candidates act-1, cdc-42, pmp-3, eif-3.C, actin, act-2, csq-1, Y45F10D.4, tba-1, mdh-1, ama-1, F35G12.2, and rbd-1 to test their expression stability under different doses of nano-copper oxide CuO 0, 1, 10, and 50 µg-mL using RT-qPCR. Four algorithms, geNorm, NormFinder, BestKeeper, and the comparative ΔCt method, were employed to evaluate these 13 candidates expressions. As a result, tba-1, Y45F10D.4 and pmp-3 were the most reliable, which may be used as reference genes in future study of nanoparticle-induced genetic response using C.elegans.



Author: Yanqiong Zhang, Dongliang Chen, Michael A. Smith, Baohong Zhang, Xiaoping Pan

Source: http://plos.srce.hr/



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