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Veterinary Archives, Vol.79 No.4 July 2009. -

Dot-blot enzyme immunoassay DB-EIA was utilized for the detection of bovine viral diarrhea BVD antibodies in infected cattle. In this assay whole particles of the NADL strain of BVD virus were used as an antigen. A total of 1250 sera from SN and ELISA tested cows were tested for BVDV antibodies. HRPO conjugated protein G was used to detect bound BVDV antibodies using TMB as the substrate. Statistical analyses did not show any significant differences in the sensitivity of DB-EIA, to the ELISA and SN tests. The assay proved to be a simple, inexpensive, reliable and rapid tool for BVD serodiagnosis.

dot-blot enzyme immunoassays; bovine viral diarrhea; serum neutralization test; ELISA



Author: Farhid Hemmatzadeh - ; Department of Microbiology, Faculty of Veterinary Science, University of Melbourne, Australia Farhad Amini

Source: http://hrcak.srce.hr/



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