Production and characterization of chitinase from Vibrio species, a head waste of shrimp Metapenaeus dobsonii Miers, 1878 and chitin of Sepiella inermis Orbigny, 1848Report as inadecuate




Production and characterization of chitinase from Vibrio species, a head waste of shrimp Metapenaeus dobsonii Miers, 1878 and chitin of Sepiella inermis Orbigny, 1848 - Download this document for free, or read online. Document in PDF available to download.

The chitin is extracted from the cuttlebone of S. inermis and the mineral contents are predictable. The structure and degree of deacetylation of extracted cuttlebone chitin is dogged through Fourier Transform Infrared FT-IR spectroscopy. The extracted cuttlebone chitin is used as the substrate for the production of chitinase from Vibrio sp. The extra cellular proteins are concentrated by ammonium sulphate precipitation, dialysed and then purified by using gel sephadex G-100 chromatography. Among the 40 fractions, only two fractions active fractions showed maximum absorbance at 280 nm, which are pooled, dialysed and free-dried. The enzyme activity 0.104 μmoles-ml and molecular weight 50 - 60 kDa of purified chitinase is also determined through SDS- PAGE. The optimal condition for chitinase activity is pH between 6.0 - 6.5 and 45℃.

KEYWORDS

Chitin; S. inermis; Chitnase; M. dobsonii; Vibrio sp.

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Revathi, M. , Saravanan, R. and Shanmugam, A. 2012 Production and characterization of chitinase from Vibrio species, a head waste of shrimp Metapenaeus dobsonii Miers, 1878 and chitin of Sepiella inermis Orbigny, 1848. Advances in Bioscience and Biotechnology, 3, 392-397. doi: 10.4236-abb.2012.34056.





Author: Masilamani Revathi, Ramachandran Saravanan, Annaian Shanmugam

Source: http://www.scirp.org/



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