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A large body of evidence shows that a single bout of strenuous exercise induces oxidative stress in circu- lating human lymphocytes leading to lipid peroxide- tion, DNA damage, mitochondrial perturbations, and protein oxidation. In a training experiment, Wistar rats were divided into control group CG and exer- cise group EG. After a running level exercise until exhaustion, we observed an increase in the mRNA content and protein expression of SIRT1 and SIRT7 in the EG compared to the CG. Moreover, such train- ing exercise did not change mRNA transcripts and protein expression of FOXO3A and GADD45. We also observed an increase of pro-apoptotic protein bax and a decrease of the anti-apoptotic protein bcl-2 in the EG. Accordingly, we observed a caspase-3 activation and poly ADP-ribose polymerase PARP cleavage only in EG rats. Statistical analysis of the data showed a significant correlation between SIRT1 and SIRT7 expression and apoptotic proteins such as bax, bcl-2 in both tissues. We conclude that, in both muscle, such exercise activates both a damaging apoptotic mecha- nism with bax increase and bcl-2 decrease and a counterbalancing protective mechanism with SIRT1 and SIRT7 increase.

KEYWORDS

Cardiac Muscle; Skeletal Muscle; Bax; Bcl-2; SIRT1; SIRT7

Cite this paper

Marfe, G. , Manzi, V. , Tafani, M. , Pucci, B. , Gambacurta, A. , Antonio Russo, M. and Sinibaldi-Salimei, P. 2012 The modulation of sirtuins and apoptotic proteins in rats after exhaustive exercise. Open Journal of Molecular and Integrative Physiology, 2, 65-74. doi: 10.4236-ojmip.2012.23010.





Author: Gabriella Marfe, Vincenzo Manzi, Marco Tafani, Bruna Pucci, Alessandra Gambacurta, Matteo Antonio Russo, Paola Sinibaldi-Salimei

Source: http://www.scirp.org/



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