An Efficient Method for Plant Regeneration from Calli of Swertia mussotii, an Endangered Medicinal HerbReport as inadecuate




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Swertia mussotii Franch is an endangered medicinal plant in the Qinghai-Tibet Plateau. Its regeneration from callus culture is very difficult. In this study, an efficient method for plant regeneration was developed from its calli. The calli derived from young stem explants of S. mussotii were cultured at two types of temperature treatments to test their efficiency of shoot regeneration. When the calli were cultured at variable temperature VT, 20℃ during the day and 10℃ at night treatment, the adventitious shoots were formed at each combination tested. However, that did not occur when the calli were cultured at constant temperature CT, 25℃ treatment. The best response 53.7% of plantlet regeneration was obtained on Murashige and Skoog MS medium containing 3 mg·l-1 6-benzylaminopurine with 0.5 mg·l-1 α-naphthaleneacetic acid in the VT treatment. The regenerated plantlets were rooted on half-strength MS medium without growth regulators. They flowered in the following subculture. The results indicate that the treatment of day-night temperature difference is a critical factor to callus differentiation in S. mussotii. This protocol can be used for conservation as well as mass propagation of this medicinal plant.

KEYWORDS

Swertia mussotii; Medicinal Plant; Callus Induction; Plant Regeneration; Day-Night Temperature Difference

Cite this paper

T. He, J. Xu, L. Yang and H. Wang -An Efficient Method for Plant Regeneration from Calli of Swertia mussotii, an Endangered Medicinal Herb,- American Journal of Plant Sciences, Vol. 3 No. 7, 2012, pp. 904-908. doi: 10.4236-ajps.2012.37107.





Author: Tao He, Jing Xu, Lina Yang, Haitao Wang

Source: http://www.scirp.org/



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