The Molecular Assembly of Amyloid Aβ Controls Its Neurotoxicity and Binding to Cellular ProteinsReport as inadecuate




The Molecular Assembly of Amyloid Aβ Controls Its Neurotoxicity and Binding to Cellular Proteins - Download this document for free, or read online. Document in PDF available to download.

Accumulation of β-sheet-rich peptide Aβ is strongly associated with Alzheimer-s disease, characterized by reduction in synapse density, structural alterations of dendritic spines, modification of synaptic protein expression, loss of long-term potentiation and neuronal cell death. Aβ species are potent neurotoxins, however the molecular mechanism responsible for Aβ toxicity is still unknown. Numerous mechanisms of toxicity were proposed, although there is no agreement about their relative importance in disease pathogenesis. Here, the toxicity of Aβ 1–40 and Aβ 1–42 monomers, oligomers or fibrils, was evaluated using the N2a cell line. A structure-function relationship between peptide aggregation state and toxic properties was established. Moreover, we demonstrated that Aβ toxic species cross the plasma membrane, accumulate in cells and bind to a variety of internal proteins, especially on the cytoskeleton and in the endoplasmatic reticulum ER. Based on these data we suggest that numerous proteins act as Aβ receptors in N2a cells, triggering a multi factorial toxicity.



Author: Claudia Manzoni, Laura Colombo, Paolo Bigini, Valentina Diana, Alfredo Cagnotto, Massimo Messa, Monica Lupi, Valentina Bonetto, M

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents