Effect of Recombinant Human Erythropoietin on Survivin Expression in Brain Tissues after Traumatic Brain Injury in RatsReport as inadecuate




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Objective: To explore the regulative effect on Survivin of r-HuEPO after traumatic brain injury TBI in rats, and understand the neuroprotection mechanisms of r-HuEPO. Methods: Seventy-eight adult Wistar rats were randomly divided into sham operation group n = 6, TBI group n = 36 and r-HuEPO group n = 36. The experimental TBI model was created by Feeney’s method. Samples were obtained after injury for measuring apoptosis of cells by Epics XL Flow Cytometer. Immunochemical method was performed for inspection of expressions of Survivin and NF-κB proteins. Results: Compared to the sham group, the number of apoptotic cells and Survivin, NF-κB immunopositive cells was significantly increased in the injured brain after TBI P < 0.01. R-HuEPO significantly increased the expression of survivin and NF-κB, but decreased the apoptotic rates. Conclusion: Increased expression of NF-κB by r-HuEPO may play important role in regulating Survivin level, inhibiting neuronal apoptosis in cortex and exerting protective function to neurons.

KEYWORDS

Recombinant Human Erythropoietin r-HuEPO; Brain Injury; Survivin; NF-κB; Apoptosis

Cite this paper

P. Lei, L. Peng and X. Zhang -Effect of Recombinant Human Erythropoietin on Survivin Expression in Brain Tissues after Traumatic Brain Injury in Rats,- Surgical Science, Vol. 3 No. 5, 2012, pp. 266-270. doi: 10.4236-ss.2012.35053.





Author: Peng Lei, Longfeng Peng, Xingchao Zhang

Source: http://www.scirp.org/



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