Molecular Diversity of Kenyan Lablab Bean Lablab purpureus L. Sweet Accessions Using Amplified Fragment Length Polymorphism MarkersReport as inadecuate




Molecular Diversity of Kenyan Lablab Bean Lablab purpureus L. Sweet Accessions Using Amplified Fragment Length Polymorphism Markers - Download this document for free, or read online. Document in PDF available to download.

Lablab purpureus L. Sweet is a multipurpose legume that combines use as human food and animal feed in addition to serving as a cover crop for soil conservation. In this work, molecular diversity in Lablab purpureus was assessed using amplified fragment length polymorphism markers on fifty Kenyan lablab accessions obtained from farmers’ fields and the Kenya National gene bank. One hundred and eighty polymorphic bands were revealed using fifteen selective primer pairs. The overall mean expected heterozygosity He for the five populations was 0.189. Estimates of components of molecular variance revealed that most of the genetic variation resided within populations 99% and only 1% variance was among the populations, while Principal Coordinate Analysis showed an overlap between accessions from different geographic origins. The UPGMA cluster analysis generated from the distance matrix of the 50 assayed accessions, revealed low diversity among most of the accessions. The low diversity observed may be due to the narrow genetic base for breeding stocks, and extensive exchange of germplasm among smallholder farmers across the country. Results obtained from this study are discussed in light of the need to enhance the genetic management and improvement of this multipurpose crop species.

KEYWORDS

Lablab purpureus; Molecular Markers; AFLP; Genetic Diversity

Cite this paper

E. N. Kimani, F. N. Wachira and M. G. Kinyua -Molecular Diversity of Kenyan Lablab Bean Lablab purpureus L. Sweet Accessions Using Amplified Fragment Length Polymorphism Markers,- American Journal of Plant Sciences, Vol. 3 No. 3, 2012, pp. 313-321. doi: 10.4236-ajps.2012.33037.





Author: Esther N. Kimani, Francis N. Wachira, Miriam G. Kinyua

Source: http://www.scirp.org/



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