Enzyme-Linked Immunosorbent Assay-Format Tissue Culture Infectious Dose-50 Test for Titrating Dengue VirusReport as inadecuate




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A dengue nonstructural protein 1 NS1 antigen capture enzyme-linked immunosorbent assay ELISA-based tissue culture infectious dose-50 TCID50 test TCID50-ELISA was developed as an alternative to the standard plaque assay for titrating dengue virus. Virus titers obtained by TCID50-ELISA were comparable to those obtained by the plaque assay and by the traditional TCID50-cytopathic effect CPE test TCID50-CPE, with a better reproducibility and a lower coefficient of variation. Quantitative comparison of TCID50-ELISA and TCID50-CPE resulted in a correlation coefficient of 0.976. Moreover, this new method showed a wider application to C6-36, Vero E6, BHK-21, and Vero cells compared with other titration methods. In summary, the novel TCID50-ELISA method described here provides a more reliable and more accurate alternative compared to the plaque assay and TCID50-CPE for titration of dengue virus.



Author: Jie Li, Dong-mei Hu, Xi-xia Ding, Yue Chen, Yu-xian Pan, Li-wen Qiu, Xiao-yan Che

Source: http://plos.srce.hr/



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