BDNF Facilitates L-LTP Maintenance in the Absence of Protein Synthesis through PKMζReport as inadecuate




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Late-phase long term potentiation L-LTP is thought to be the cellular basis for long-term memory LTM. While LTM as well as L-LTP is known to depend on transcription and translation, it is unclear why brain-derived neurotrophic factor BDNF could sustain L-LTP when protein synthesis is inhibited. The persistently active protein kinase ζ PKMζ is the only molecule implicated in perpetuating L-LTP maintenance. Here, in mouse acute brain slices, we show that inhibition of PKMζ reversed BDNF-dependent form of L-LTP. While BDNF did not alter the steady-state level of PKMζ, BDNF together with the L-LTP inducing theta-burst stimulation TBS increased PKMζ level even without protein synthesis. Finally, in the absence of de novo protein synthesis, BDNF maintained TBS-induced PKMζ at a sufficient level. These results suggest that BDNF sustains L-LTP through PKMζ in a protein synthesis-independent manner, revealing an unexpected link between BDNF and PKMζ.



Author: Fan Mei, Guhan Nagappan, Yang Ke, Todd C. Sacktor, Bai Lu

Source: http://plos.srce.hr/



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