Transgenic Zebrafish Recapitulating tbx16 Gene Early Developmental ExpressionReport as inadecuate




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We describe the creation of a transgenic zebrafish expressing GFP driven by a 7.5 kb promoter region of the tbx16 gene. This promoter segment is sufficient to recapitulate early embryonic expression of endogenous tbx16 in the presomitic mesoderm, the polster and, subsequently, in the hatching gland. Expression of GFP in the transgenic lines later in development diverges to some extent from endogenous tbx16 expression with the serendipitous result that one line expresses GFP specifically in commissural primary ascending CoPA interneurons of the developing spinal cord. Using this line we demonstrate that the gene mafba valentino is expressed in CoPA interneurons.



Author: Simon Wells , Svanhild Nornes, Michael Lardelli

Source: http://plos.srce.hr/



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