Viral Etiologies of Acute Respiratory Infections among Hospitalized Vietnamese Children in Ho Chi Minh City, 2004–2008Report as inadecuate




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Background

The dominant viral etiologies responsible for acute respiratory infections ARIs are poorly understood, particularly among hospitalized children in resource-limited tropical countries where morbidity and mortality caused by ARIs are highest. Improved etiological insight is needed to improve clinical management and prevention.

Objectives

We conducted a three-year prospective descriptive study of severe respiratory illness among children from 2 months to 13 years of age within the largest referral hospital for infectious diseases in southern Vietnam.

Methods

Molecular detection for 15 viral species and subtypes was performed on three types of respiratory specimens nose, throat swabs and nasopharyngeal aspirates using a multiplex RT-PCR kit Seeplex™ RV detection, Seegene and additional monoplex real-time RT-PCRs.

Results

A total of 309 children were enrolled from November 2004 to January 2008. Viruses were identified in 72% 222-309 of cases, including respiratory syncytial virus 24%, influenza virus A and B 17%, human bocavirus 16%, enterovirus 9%, human coronavirus 8%, human metapneumovirus 7%, parainfluenza virus 1–3 6%, adenovirus 5%, and human rhinovirus A 4%. Co-infections with multiple viruses were detected in 20% 62-309 of patients. When combined, diagnostic yields in nose and throat swabs were similar to nasopharyngeal aspirates.

Conclusion

Similar to other parts in the world, RSV and influenza were the predominant viral pathogens detected in Vietnamese hospitalized children. Combined nasal and throat swabs are the specimens of choice for sensitive molecular detection of a broad panel of viral agents. Further research is required to better understand the clinical significance of single versus multiple viral coinfections and to address the role of bacterial co-infections involved in severe respiratory illness.



Author: Anh Ha Lien Do , H. Rogier van Doorn, My Ngoc Nghiem, Juliet E. Bryant, Thanh Hang thi Hoang, Quang Ha Do, Tan Le Van, Tan Thanh

Source: http://plos.srce.hr/



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