Prevalence of Human Papillomavirus Genotypes in Cervical Cancer and Precursor LesionsReport as inadecuate

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Collegium antropologicum, Vol.30 No.4 December 2006. -

There are no data obtained in biopsy material on the prevalence of human papillomavirus HPV and HPV genotypes

in Croatian women with cervical carcinoma and precursor lesions. Therefore, the prevalence of HPV and HPV genotypes

was investigated in archival material of cervical carcinoma and precursor lesions kept at Department of Pathology,

School of Medicine, University of Rijeka. DNA was isolated from formalin fixed, paraffin embedded tissue, histologically

classified as cervical intraepithelial neoplasia CIN III n=43, squamous cell carcinoma SCC n=54 and adenocarcinoma

ADC n=40. HPV testing was performed by polimerase chain reaction PCR using generic and genotype

specific primers. The prevalence of HPV DNA was 93.02%, 92.59%, and 92.5% in CIN III, SCC and ADC, respectively. In

CIN III and SCC, HPV-16 was the most common high-risk genotype, identified in 65% and 52%, followed by HPV-18 in

22.5% and 28% of cases, respectively. HPV-18 showed a statistically significant prevalence in ADC 67.6% as compared

with SCC 2=9.924; p 0.01. Study results revealed a high prevalence of HPV-DNA in examined cervical lesions >90%.

HPV-16 predominated in SCC and HPV-18 in ADC. Single infection was more frequently present than multiple infections

in all three histological groups.

cervical intraepithelial neoplasia; cervical carcinoma; HPV genotype prevalence

Author: Ita Hadžisejdić - Marina Šimat - Ana Bosak - Maja Krašević - Blaženka Grahovac -



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