Polyamines Are Present in Mast Cell Secretory Granules and Are Important for Granule HomeostasisReport as inadecuate




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Background

Mast cell secretory granules accommodate a large number of components, many of which interact with highly sulfated serglycin proteoglycan PG present within the granules. Polyamines putrescine, spermidine and spermine are absolutely required for the survival of the vast majority of living cells. Given the reported ability of polyamines to interact with PGs, we investigated the possibility that polyamines may be components of mast cell secretory granules.

Methodology-Principal Findings

Spermidine was released by mouse bone marrow derived mast cells BMMCs after degranulation induced by IgE-anti-IgE or calcium ionophore A23187. Additionally, both spermidine and spermine were detected in isolated mouse mast cell granules. Further, depletion of polyamines by culturing BMMCs with α-difluoromethylornithine DFMO caused aberrant secretory granule ultrastructure, impaired histamine storage, reduced serotonin levels and increased β-hexosaminidase content. A proteomic approach revealed that DFMO-induced polyamine depletion caused an alteration in the levels of a number of proteins, many of which are connected either with the regulated exocytosis or with the endocytic system.

Conclusions-Significance

Taken together, our results show evidence that polyamines are present in mast cell secretory granules and, furthermore, indicate an essential role of these polycations during the biogenesis and homeostasis of these organelles.



Author: Gianni García-Faroldi, Carlos E. Rodríguez, José L. Urdiales, José M. Pérez-Pomares, José C. Dávila, Gunnar Pejler, Franci

Source: http://plos.srce.hr/



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