Nutritional Requirements for the Mycelial Biomass and Exopolymer Production by Hericium erinaceus CZ-2Report as inadecuate




Nutritional Requirements for the Mycelial Biomass and Exopolymer Production by Hericium erinaceus CZ-2 - Download this document for free, or read online. Document in PDF available to download.

Food Technology and Biotechnology, Vol.45 No.4 December 2007. -

In this work, the effects of medium composition and fermentation parameters on the simultaneous production of mycelial biomass and exopolymer by medicinal mushroom Hericium erinaceus CZ-2 were investigated in shake flask cultures using one-factor-at-a-time method and orthogonal array design. Results showed that the most suitable carbon, nitrogen, mineral sources, and cofactors for the mycelial biomass and exopolymer production were: corn flour combined with 1 % glucose, yeast extract, KH2PO4 and corn steep liquor. The intuitive analysis of orthogonal array design results indicated that the effects of nutritional requirement on the mycelial growth of Hericium erinaceus CZ-2 were in regular sequence of corn flour combined with 1 % glucose > yeast extract > corn steep liquor > KH2PO4, and those on exopolymer production were in the order of corn flour combined with glucose > KH2PO4 > yeast extract > corn steep liquor. The maximal yield of mycelial biomass 16.07 g-L was obtained when the composition of the culture medium was in g-L: corn flour 30, glucose 10, yeast extract 3, KH2PO4 1, CaCO3 0.5, and 15 mL-L of corn steep liquor; while the maximal exopolymer yield 1.314 g-L was achieved when the composition of medium was in g-L: corn flour 30, glucose 10, yeast extract 5, KH2PO4 3, CaCO3 0.5, and 15 mL-L of corn steep liquor. In the 15-litre scale-up fermentation, the maximum mycelial biomass yield of 20.50 g-L was achieved using the optimized medium.

Hericium erinaceus; submerged culture; medium optimization; mycelial biomass; exopolymer; orthogonal array design method



Author: Daming Huang - ; Institute of Bioengineering, School of Food and Biological Technology, Jiangsu University, 212013 Zhenjiang, PR

Source: http://hrcak.srce.hr/



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