Substituting Dietary Polyunsaturated Fat with Monounsaturated Fat Increases Insulin Sensitivity in Cultured Rat HepatocytesReport as inadecuate




Substituting Dietary Polyunsaturated Fat with Monounsaturated Fat Increases Insulin Sensitivity in Cultured Rat Hepatocytes - Download this document for free, or read online. Document in PDF available to download.

Collegium antropologicum, Vol.32 No.1 May 2008. -

Increased dietary fat intake generally, and saturated fat specifically, will lead to impairment of insulin action and to

development of metabolic syndrome. The aim of this study was to find out changing of hepatic glucose output in dependence

of high monounsaturated and high polyunsaturated fat diet and possible direct action of insulin in hepatocytes.

Hepatocytes were isolated by a collagenase perfusion technique and cultured for 24 h in M 199 serum-free medium. The

glucose production in hepatocytes isolated from rats on high polyunsaturated fat diet, as well as those isolated from rats

on high monounsaturated fat diet was significantly higher than in standard controls. Insulin significantly decreased

glucose production but only in hepatocytes obtained from rats on high monounsaturated fat diet. On the other side energy

expenditure was significantly higher in rats on high monounsaturated fat diet and significantly lower in rats on high

polyunsaturated fat diet comparable with animals on standard diet. Monounsaturated fat in Mediterranean diet could

have beneficial effect on the development of the metabolic syndrome by increasing insulin sensitivity and energy expenditure.

insulin; insulin resistance; glucose production; gluconeogenesis; metabolic syndrome



Author: Jagoda Roša - Anja Baraba - Petra Bučević - Darija Đorđijevski - Ivan Antolković - Josip Roša -

Source: http://hrcak.srce.hr/



DOWNLOAD PDF




Related documents