Rapid PCR Detection of Salmonella in Animal Feces SamplesReport as inadecuate




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The study intended to establish a rapid, sensitive and accurate detection technique for Salmonella in animal feces. A set of primers was devised according to stn gene in Salmonella to detect Salmonella of various serovars by PCR amplification. The sensitivity and accuracy of this method and the detection limit in the artificial feces samples were studied. While we used this method to detect Salmonella in 100 feces samples, conventional culture technique was used to demonstrate the validity of this method. The primers used in this experiment were specific to Salmonella and the band of 260 bp was obtained. The 43.85 pg DNA of Salmonella could be detected by PCR. The detection sensitivity was 1 cfu -ml in pure culture. The detection limit of artificially contaminated feces samples was 103 cfu -ml without using enrichment procedure while the detection limit was 1 cfu -ml using enrichment procedure. The PCR method detected Salmonella in 18 out of the 250 samples, and conventional culture technique supported the result. Thus the stn primers are specific for Salmonella species and the PCR method presented may be suitable for the detection of Salmonella in feces.

KEYWORDS

Salmonella; stn; PCR; feces

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Author: Zhiming Pan, Yanqing Zhou, Beibei Liu, Xinan Jiao

Source: http://www.scirp.org/



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