A Proteomic Approach for the Diagnosis of Bacterial MeningitisReport as inadecuate




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Background

The discrimination of bacterial meningitis BM versus viral meningitis VM shapes up as a problem, when laboratory data are not equivocal, in particular, when Gram stain is negative.

Methodology-Principal Findings

With the aim to determine reliable marker for bacterial or viral meningitis, we subjected cerebrospinal fluid CSF to a quantitative proteomic screening. By using a recently established 2D-DIGE protocol which was adapted to the individual CSF flow, we compared a small set of patients with proven BM and VM. Thereby, we identified six potential biomarkers out of which Prostaglandin-H2 D-isomerase was already described in BM, showing proof of concept. In the subsequent validation phase on a more comprehensive collective of 80 patients, we could validate that in BM high levels of glial fibrillary acidic protein GFAP and low levels of soluble amyloid precursor protein alpha-beta sAPPα-β are present as possible binding partner of Fibulin-1.

Conclusions-Significance

We conclude that our CSF flow-adapted 2D-DIGE protocol is valid especially in comparing samples with high differences in total protein and suppose that GFAP and sAPPα-β have a high potential as additional diagnostic markers for differentiation of BM from VM. In the clinical setting, this might lead to an improved early diagnosis and to an individual therapy.



Author: Sarah Jesse, Petra Steinacker, Stefan Lehnert, Martin Sdzuj, Lukas Cepek, Hayrettin Tumani, Olaf Jahn, Holger Schmidt, Markus Ott

Source: http://plos.srce.hr/



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