miR-24 Regulates Apoptosis by Targeting the Open Reading Frame ORF Region of FAF1 in Cancer CellsReport as inadecuate




miR-24 Regulates Apoptosis by Targeting the Open Reading Frame ORF Region of FAF1 in Cancer Cells - Download this document for free, or read online. Document in PDF available to download.

Background

microRNAs miRNAs are small noncoding RNAs that regulate cognate mRNAs at the post-transcriptional stage. Several studies have shown that miRNAs modulate gene expression in mammalian cells by base pairing to complementary sites in the 3′-untranslated region 3′-UTR of the target mRNAs.

Methodology-Principal Findings

In the present study, miR-24 was found to target fas associated factor 1FAF1 by binding to its amino acid coding sequence CDS region, thereby regulating apoptosis in DU-145 cells. This result supports an augmented model whereby animal miRNAs can exercise their effects through binding to the CDS region of the target mRNA. Transfection of miR-24 antisense oligonucleotide miR-24-ASO also induced apoptosis in HGC-27, MGC-803 and HeLa cells.

Conclusions-Significance

We found that miR-24 regulates apoptosis by targeting FAF1 in cancer cells. These findings suggest that miR-24 could be an effective drug target for treatment of hormone-insensitive prostate cancer or other types of cancers. Future work may further develop miR-24 for therapeutic applications in cancer biology.



Author: Wenming Qin, Yi Shi, Botao Zhao, Chengguo Yao, Li Jin, Jiexian Ma, Youxin Jin

Source: http://plos.srce.hr/



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